ISSN:
1573-4978
Keywords:
autoimmunity
;
connective tissue disease
;
B cell epitope
;
Topoisomerase I
;
autoantibodies
;
autoantigen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary In this paper an immunodominant epitope of Topoisomerase I is described. An epitope expression sublibrary was constructed from Topoisomerase I cDNA. The subclones were screened with an antiserum from a patient with systemic sclerosis (SSc). The positive clones defined one immunodominant B cell epitope (epitope III), which was located at the carboxyterminal part of the protein. The epitope, 52 amino acids in length, neither contains the p 30gag sequence nor the suggested active site Tyr-723, both presumed antibody recognition sites. More than 70% of our anti-TopoI sera recognize this epitope III, indicating that it is a major recognition site of the anti-TopoI autoantibodies in SSc sera. DNA relaxation experiments show that all sera that recognize epitope III and most sera with antibodies to other epitopes inhibit Topoisomerase I activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00419757
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