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  • 1
    Electronic Resource
    Electronic Resource
    Pulsing of multiple nutrients as a strategy to achieve large biologically active zones during in situ carbon tetrachloride remediation (1997)
    Springer
    Computational geosciences 1 (1997), S. 271-288 
    Details
    ISSN: 1573-1499
    Keywords: bioremediation ; numerical simulation ; carbon tetrachloride ; denitrification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Computer Science
    Notes: Abstract When injecting nutrients during active bioremediation, various nutrient addition strategies can be employed in order to remediate the site at minimal expense. In particular, if more than one nutrient is required, a pulse addition strategy can be devised in which these nutrients are added as temporally separated pulses such that the biostimulated portion of the flow field can be maximized. In this manuscript, results of numerical simulations are reported which demonstrate this effect. Previously published kinetic expressions describing microbial growth and carbon tetrachloride degradation under denitrifying conditions are used in these simulations. The simulations indicate that volumetric treatment can be accomplished by injecting long duration nutrient pulses of different nutrients that are separated by a long period where only unamended water is injected. This strategy will cause the nutrient pulses to overlap at a considerable distance from the injection point, while maintaining concentrations that are high enough to ensure significantbiomass growth and contaminant destruction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011573429996
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biological destruction of CCl4: I. Experimental design and data (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 43 (1994), S. 521-528 
    Details
    ISSN: 0006-3592
    Keywords: carbon tetrachloride ; acetate ; nitrate ; bioremediation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A denitrifying consortium capable of transforming carbon tetrachloride (CCl4) was cultured from aquifer sediment from the U.S. Department of Energy's Hanford Site in southeastern Washington State. To understand the kinetics of the biological destruction of CCl4 by these microbes, a set of experiments, the conditions of which were chosen according to a fractional factorial experimental design, were completed. This article reports on the experimental design along with the results for CCl4, biomass, acetate, nitrate, and nitrite concentrations. These data indicate that growth is inhibited by high nitrite concentrations, whereas CCl4 degradation is slowed by the presence of nitrate and/or nitrite. © 1994 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260430613
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of nitrate and acetate availability on chloroform production during carbon tetrachloride destruction (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 551-557 
    Details
    ISSN: 0006-3592
    Keywords: denitrify ; carbon tetrachloride ; chloroform ; acetate ; nitrate ; bioremediation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fed batch experiments were performed to test the effects of electron donor and electron acceptor availability on the production of chloroform (CF) during carbon tetrachloride (CT) destruction by a denitrifying bacterial consortium. In one series of tests, acetate (electron donor) was present in excess while nitrate and nitrite (electron acceptor) were limiting. In the other series of tests, acetate was the limiting nutrient, and nitrate and nitrite were in excess. Under nitrate limiting conditions, 50% (±17%) of the CT transformed by the microorganisms was converted to CF. However, under acetate limiting conditions, only 4% (±4%) of the CT that was degraded appeared as CF. Previous research had suggested that denitrifying bacteria can degrade CT via two competing pathways. One of these pathways produces CF as the predominant end product. The second pathway produces CO2 as the primary end product. The results shown here suggest that the first pathway is dominant when nitrate and nitrite are depleted while the second pathway, which produces little CF, dominates when nitrate or nitrite are available.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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