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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 43 (1996), S. 343-349 
    ISSN: 1573-5109
    Keywords: duplication ; bulking ; Brassica oleracea ; Brussels sprouts ; white cabbage ; genetic resources ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To reduce the number of accessions in the Brassica oleracea collection of the Centre for Genetic Resources The Netherlands (CGN) groups of accessions were bulked. Accessions in a group were selections from the same landrace or old variety, and were chosen, with the help of crop experts, on the basis of their history and morphology. Since this approach was received rather sceptically in the genebank community, a genetical analysis of some of the groups and their components (named varieties) was made to justify the procedures. A selection of white cabbage and Brussels sprouts, representing two crop types of B. oleracea, was made: eleven white cabbage groups comprising 43 components, and nine Brussels sprouts groups comprising 24 components. Each group and all of its components were described on the basis of electrophoresis of nine isoenzymes on 30 plants. Most components were rightly classified; 16 percent were misclassified. All these misclassifications were within similar groups. It could be concluded that pooling of the components included in this study was justified. In two cases the isozymic patterns suggested that the groups could even have been larger. Only in the case of the two groups of Brussels sprouts ‘Bedfordshire’ this was a real option. In the other case involving the white cabbage groups ‘Langedijker Bewaar Gewoon’, ‘Langedijker Bewaar Graag’ and ‘Late Herfstdeen’ this was not an option since these landraces, although having a common genetic background, clearly have a distinct identity as defined by morphology and history.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of assisted reproduction and genetics 10 (1993), S. 37-43 
    ISSN: 1573-7330
    Keywords: in vitro fertilization ; embryo transfer technique ; cervix dilatation ; pregnancy rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Results The following observations were made. Retention of embryos in the transfer sets significantly reduced the pregnancy rate (P =0.015); catheters contaminated with blood and cervical mucus indirectly contributed to this effect by increasing the incidence of failed first transfer attempts. Even though cervical dilatations, if indicated by uterus sounding, were done 2 days before embryo transfer, no pregnancies were effected in these 18 cases (P =0.0001). Late transfers of embryos, due to delayed fertilization or slow cleavage rates, yielded a pregnancy rate of 10.5%.
    Abstract: Conclusion The approach of immediately retransferring retained embryos does not solve the problem of reduced pregnancy rates in FFA cases. It is suggested that ET should be repeated 1 day later in FFA cases in an attempt to improve pregnancy rates.
    Notes: Purpose This study derives from the observation that a correlation exists between failed first attempts (FFA) at embryo transfer caused by one or more embryos remaining in the catheter and reduced pregnancy rates (20.3 vs 3.0%). The aim of this study was to examine the relationship between failed first attempts at transfer and contamination of the transfer set; the related aspects of cervix dilatation and late embryo transfer were also investigated.
    Type of Medium: Electronic Resource
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