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  • 1
    Electronic Resource
    Electronic Resource
    Two closely related cDNAs encoding starch branching enzyme from Arabidopsis thaliana (1996)
    Springer
    Plant molecular biology 30 (1996), S. 97-108 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; cDNA ; gene expression ; starch biosynthesis ; starch branching enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two starch branching enzyme (SBE) cDNAs were identified in an Arabidopsis seedling hypocotyl library using maize Sbe1 and Sbe2 cDNAs as probes. The two cDNAs have diverged 5′ and 3′ ends, but encode proteins which share 90% identity over an extensive region with 70% identity to maize SBE IIb [12]. Genomic Southern blots suggest that the two cDNAs are the products of single, independent genes, and that additional, more distantly related SBE genes may exist in the Arabidopsis genome. The two cDNAs hybridize to transcripts which show similar expression patterns in Arabidopsis vegetative and reproductive tissues, including seedlings, inflorescence rachis, mature leaves, and flowers. This is the first report of the identification of cDNAs encoding two closely related starch branching enzymes from the same species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017805
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of the Amylose-Extender gene encoding starch branching enzyme IIB in maize (1998)
    Springer
    Plant molecular biology 38 (1998), S. 945-956 
    Details
    ISSN: 1573-5028
    Keywords: amylose-extender ; Ae ; genomic organization ; promoter analysis ; Zea mays L. ; starch-branching enzyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The amylose-extender (Ae) gene encoding starch-branching enzyme IIb (SBEIIb) in maize is predominantly expressed in endosperm and embryos during kernel development. A maize genomic DNA fragment (−2964 to +20 485) containing the Ae gene was isolated and sequenced. The maize Ae mRNA is derived from 22 exons distributed over 16 914 bp. Twenty-one introns, differing in length from 76 bp to 4020 bp, all have conserved junction sequences (GT⋅⋅AG). Sequence analysis of the 5′- and 3′-flanking regions revealed a consensus TATA-box sequence located 28 bp upstream of the transcription initiation site as determined by primer extension analysis, and a putative polyadenylation signal observed 29 bp upstream of the polyadenylation site based on cDNA sequence. Genomic Southern blot analysis suggests that a single Ae gene is present in the maize genome. Promoter activity was confirmed by testing a transcriptional fusion of the Ae 5′-flanking region between −2964 and +100 to a luciferase reporter gene in a transient expression assay using maize endosperm suspension cultured cells. 5′ deletion analysis revealed that the 111 bp region from −160 to −50 is essential for high-level promoter activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006057609995
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    Paper (German National Licenses)
    Fulltext
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