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  • 1
    Electronic Resource
    Electronic Resource
    Identification of an endochitinase cDNA clone from barley aleurone cells (1989)
    Springer
    Plant molecular biology 12 (1989), S. 403-412 
    Details
    ISSN: 1573-5028
    Keywords: barley ; cDNA ; chromosomal location ; endochitinase ; sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A barley aleurone cDNA library was screened using 32P-labeled cDNA prepared by reverse transcription of mRNA from aleurone layers treated in the presence or absence of gibberellic acid (GA). Besides α-amylase cDNA clones, another set of clones representing an abundant mRNA in aleurone cells was identified. Messenger RNA hybrid-selected by a prototype clone of this group (clone 10) was translated in vitro to yield a 36 kDa protein. Analysis of the DNA sequence and the predicted amino acid sequence of the protein product of this clone indicates that this gene codes for a protein with homology to endochitinases from tobacco and bean. In addition, the predicted amino acid sequence includes a stretch that is closely related to a cyanogen bromide cleavage fragment from an endochitinase isolated from barley endosperm. The structural genes for endochitinase are present as multiple copies on barley chromosome 1. mRNA detectable by this clone increases in abundance in barley aleurone cells incubated in the absence and in the presence of GA. Western blot analysis of proteins from aleurone and endosperm tissues indicates the presence of multiple endochitinases differing in molecular size. Possible mechanisms for the occurrence of multiple forms of endochitinases and their biological significance are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017580
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A novel cereal storage protein: molecular genetics of the 19 kDa globulin of rice (1992)
    Springer
    Plant molecular biology 18 (1992), S. 151-154 
    Details
    ISSN: 1573-5028
    Keywords: α-Globulin ; cDNA nucleotide sequence ; mRNA ; rice endosperm storage protein ; single-copy gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A λgt11 cDNA library, constructed from poly(A)+ RNA isolated from immature rice seed endosperm, was screened with affinity-purified antibodies against the rice storage protein called α-globulin (previously), or the 19 kDa globulin (our term). A positive clone was isolated and sequenced and shown to encode a 21 kDa precursor for the 19 kDa globulin, based on the identity of portions of the inferred amino acid sequence and the sequence of three cyanogen bromide peptides of the 19 kDa globulin. Analysis of genomic DNA by Southern blotting using the cDNA clone probe revealed one hybridizing band inEco RI,Hind III, andBam HI digests. This strongly suggests that the 19 kDa globulin is encoded by a single-copy gene. Because of its single-copy nature and its abundance of Arg and lack of Lys, the 19 kDa rice globulin appears to be a particularly attractive target for genetically engineering increased Lys content in rice seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018470
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    Paper (German National Licenses)
    Fulltext
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