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Effects of cadmium on gene expression in cadmium-tolerant and cadmium-sensitiveDatura innoxia cells (1989)

Delhaize, Emmanuel ; Robinson, Nigel J. ; Jackson, Paul J.

Springer

Plant molecular biology 12 (1989), S. 487-497

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ISSN: 1573-5028

Keywords: cadmium ; Datura innoxia ; gene expression ; heat shock ; tolerance

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The effect of Cd on gene expression in suspension cultures of twoDatura innoxia cell lines with differing Cd tolerance was studied.In vivo labeling experiments using [3H] leucine showed that Cd induced the synthesis of a similar range of proteins in both cell lines at a concentration which will kill the sensitive but not the tolerant cells. Corresponding changes in levels of translatable mRNA were also observed. The induction of the synthesis of proteins by Cd was transient since Cd-tolerant cells growing continuously in 250 μM CdCl2 contained a similar set ofin vitro translation products to cells growing in the absence of Cd. Although Cd had a similar effect on gene expression in both cell lines, Cd-tolerant cells possess two abundant mRNAs which are constitutively produced. These mRNAs encode proteins of low molecular weight (about 11 kDa) and are either absent or present at a low level in Cd-sensitive cells. The functions of these proteins are not known but they may be involved in the tolerance mechanism. Two-dimensional gel electrophoresis ofin vitro translation products showed that many of the Cd-induced proteins are also induced by heat shock. A 42°C heat shock resulted in agreater range and more intense induction of translatable mRNAs than 4 h exposure to 250 μM CdCl2. However a subset of mRNAs were induced specifically by Cd while other mRNAs were heat shock-specific. There was no difference in the ability of the two cell lines to tolerate heat shock. This was also reflected by the same pattern of major proteins induced by heat shock in the two cell lines.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00036963

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Investigations by inverse gas chromatography relevant to coated glass fibre/epoxy composites (1994)

Jackson, Paul L. ; Huglin, Malcolm B. ; Cervenka, Antonin

New York, NY [u.a.] : Wiley-Blackwell

Polymer International 35 (1994), S. 145-152

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ISSN: 0959-8103

Keywords: inverse gas chromatography ; cured epoxy resin ; solubility parameter ; coated glass fibre ; composite ; interaction parameter λ ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: Inverse gas chromatography has been used on two types of coated glass fibre over a range of temperatures. Interaction parameters, χ, extrapolated to 298K yielded values for the solubility parameter, δ, of 20.1 (MPa)1/2 and 23.5(MPa)1/2. After removal by combustion of the coatings, a higher value was yielded for the δ of glass. The findings, in conjunction with values of 19.8(MPa)1/2 and 20-6(MPa)1/2 for an amine cured epoxy resin and phthalic anhydride cured epoxy resin, respectively, provide a useful guide to the appropriate combination of cured resin matrix with coating with respect to optimum interfacial interaction.

Additional Material: 5 Ill.