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  • 1
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in Simarouba glauca (1994)
    Springer
    Plant cell, tissue and organ culture 37 (1994), S. 79-81 
    Details
    ISSN: 1573-5044
    Keywords: callus ; in vitro ; somatic embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Frequency of somatic embryogenesis from callus cultures derived from immature cotyledon explants of Simarouba glauca Linn. was highest on solid MS medium supplemented with 11.1 μM benzyladenine and 13.42 μM α-naphthaleneacetic acid. On transfer of the somatic embryos into maturation medium containing half-strength MS medium supplemented with 1.89 μM abscisic acid (ABA) and 2% (w/v)sucrose, 20–25 % of embryos germinated within 20 days of culture with distinct cotyledon, hypocotyl and radicle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048121
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in callus cultures of Rosa hybrida L. cv. Landora (1991)
    Springer
    Plant cell, tissue and organ culture 27 (1991), S. 65-69 
    Details
    ISSN: 1573-5044
    Keywords: callus ; in vitro ; L-proline ; Rosa hybrida cv. Landora ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus was initiated from immature leaf and stem segments of rose (Rosa hybrida cv. Landora) and subcultured every four weeks on a basal medium of half-strength Murashige & Skoog (1962) salts plus 30 g l-1 sucrose (1/2 MS) and supplemented with 2.2 μM BA, 5.4 μM NAA and 2.2–9.0 μM 2,4-D. Embryogenic callus and subsequently somatic embryos were obtained from 8-week-old callus culture on 1/2 MS+2.2 μM BA+0.05 μM NAA+0.3 μM GA3+200−800 mg l-1 L-proline. Long-term cultures were established and maintained for up to 16 months by repeated subculture of embryogenic callus on L-proline deficient medium. About 12% of cotyledonary stage embryos taken from cultures cold-stored at 8±1°C for 4 days germinated on 1/2 MS+2.2 μM BA+0.3 μM GA3+24.7 μM adenine sulphate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048208
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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