ISSN:
1040-7685
Keywords:
micellar electrokinetic capillary chromatography
;
capillary electrophoresis
;
micellar separations
;
resolution
;
mixed micelles
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The effect of surfactant concentration on resolution in micellar electrokinetic capillary chromatography (MECC) was investigated using several phenylthiohydantoin (PTH) derivatized amino acids as test solutes. Two aqueous micellar systems were employed: sodium dodecyl sulfate (SDS) and a mixed micellar system consisting of SDS and polyoxyethylene(23)dodecanol (Brij® 35). In the SDS system, the tmc/to ratio (elution range) increased significantly with increasing surfactant concentration, making the determination of the optimal concentration, which is dependent on tmc/to, difficult. In contrast, in the Brij 35/SDS system the tmc/to ratio decreased only slightly with increasing concentration of Brij 35. Whereas the SDS and Brij 35/SDS systems are better suited for moderately hydrophobic and hydrophilic solutes, respectively, neither is well-suited for hydrophobic solutes without organic modifiers. The narrower elution range (lower tmc/to) observed for the Brij 35/SDS micellar system was compensated by several potentially important advantages over the pure SDS system, including significantly higher efficiencies (2-3 times) and lower operating currents. Differences in selectivity and retention mechanisms among solutes and surfactant systems are also discussed.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220040206
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