ISSN:
1420-9071
Keywords:
Fibroblasts
;
cell culture
;
protein synthesis
;
isaxonine
;
collagen
;
colchicine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Human skin fibroblasts in confluent cultures were incubated for 24 h in the presence of isaxonine phosphate (Nerfactor) and several related factors. The incorporation of14C-proline into secreted proteins and the release of collagen into the medium were inhibited. When the cells incubated for an additional period of 24 h after thorough washing, protein and collagen syntheses were found to be identical to those of controls, demonstrating that the inhibition of protein synthesis was independent of any toxic effect. When cells were incubated in the presence of both isaxonine and colchicine, the secretion of collagen was more inhibited than by colchicine alone, and proteins accumulated in the cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01942517
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