ISSN:
1040-7685
Keywords:
isoelectric focusing
;
cone shaped capillaries
;
pH gradient
;
temperature gradient
;
proteins
;
absorption imaging detection
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A simple method of generating pH gradients for use in capillary isoelectric focusing was described and evaluated experimentally. In the proposed scheme, the cone shaped capillary filled with a conventional buffer is used to produce an effect of nonuniform heating which then generate temperature and corresponding pH gradients along the capillary axis. Concentration and separation of proteins by applying a simple isoelectric focusing system based on this principle is demonstrated. An inexpensive absorption imaging detection system is used to continuously monitor progress of the separation and concentration processes.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220050502
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