ISSN:
1573-7330
Keywords:
in vitro fertilization
;
human oocytes
;
cytogenetic analysis
;
parthenogenetic activation
;
puromycin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Setting The setting was our IVF laboratory.
Abstract:
Methods Eighty-two spare oocytes from 34 IVF patients were used. In the first series of experiments 39 unfertilized oocytes were cultured in medium containing 100, 50, or 10 µg/ml puromycin for 6 to 24 hr. After the appearance of pronuclei they were transferred to plain medium, further cultured, and cytogenetically analyzed. In the second series of experiments 43 oocytes were cultured for 5 to 10 hr in 10 µg/ml puromycin, transferred to plain medium, and fixed for cytogenetic analysis 2 hr after nuclear envelope breakdown.
Notes:
Objective Our objective was to obtain parthenogenetic activation of unfertilized human oocytes by puromycin and to try to use this procedure for cytogenetic purposes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01203955
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