ISSN:
1573-8221
Keywords:
intestine
;
choleragen
;
dehydration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A method of titration of choleragen in tadpoles ofRana temporaria is suggested. Unlike in methods known hitherto, the animals are kept (10 at a time) in vessels with water containing serial dilutions of choleragen. The degree of activity of the choleragen is determined from the percentage of dying animals. The dilution of cholergen causing death of 50% of tadpoles after 24 h was taken as the unit of activity. The method is simple, accurate, easily reproducible, and economical in use.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00835178
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