ISSN:
1573-904X
Keywords:
ultrasound
;
sonophoresis
;
transdermal
;
diagnostics
;
interstitial fluid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. Transdermal extraction of clinically relevant analytes offersa potentially non-invasive method of diagnostics. However,development of such a method is limited by the low skin permeability. In thispaper, we report a potential method for non-invasive diagnostics basedon ultrasonic skin permeabilization and subsequent extraction ofinterstitial fluid (ISF) across the skin.Methods. In vivo experiments were performed using Sprague Dawleyrats to assess ultrasound-induced skin permeabilization and subsequentextraction of various analytes. Serum and ISF concentrations of variousanalytes were measured.Results. Application of low-frequency ultrasound rapidly increasedskin permeability. Skin remained in a state of high permeability for atleast three hours. During this period, application of vacuum extractedISF across rat skin in vivo at a rate of 25.7 μl/cm2/hr. We measuredconcentrations of various analytes including glucose, albumin, calcium,urea, triglycerides, lactate, and dextran in transdermally extracted fluid.The composition of the fluid extracted transdermally is similar to thatof ISF.Conclusions. Application of low-frequency ultrasound allows skin permeabilization and extraction of ISF across the skin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007537222591
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