ISSN:
1573-8221
Keywords:
5-bromodeoxyuridine
;
chromosomes
;
differential staining
;
DNA replication
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Cultures of peripheral blood lymphocytes from normal persons of both sexes were treated with 5-bromodeoxyuridine (BDU; 20 μg/ml), 5-fluorodeoxyuridine (FDU; 0.1 μg/ml), and uridine (2 μg/ml). Chromosome preparations were obtained 5 h later, incubated in a solution of the fluorochrome Hoechst 33258, and then stained with a 2% solution of Giemsa's stain in phosphate buffer, pH 5.0. Segments of the chromosomes that incorporated BDU stained weakly with Giemsa's stain, as a result of which the late-replicating regions showed up in each chromosome as weakly stained segments. The late replication pattern of DNA in all human chromosomes was described on the basis of this method. The method is much superior in resolving power to autoradiography.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00800180
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