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  • 1
    Electronic Resource
    Electronic Resource
    Embryo density and medium volume effects on early murine embryo development (1992)
    Springer
    Journal of assisted reproduction and genetics 9 (1992), S. 454-457 
    Details
    ISSN: 1573-7330
    Keywords: embryo culture ; density ; culture volume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Methods Embryos were collected from immature C57BL6 female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by CD1 males. Groups of 1, 5, 10, or 20 embryos were cultured in 5-, 10-, 20-, or 40-µl drops of CZB under silicon oil at 37.5°C in a humidified atmosphere of 5% CO 2 and 95% air.
    Abstract: Results Development score for embryos cultured in 10 µl was higher than that of embryos cultured in 20 or 40 µl. Embryos cultured in groups of 5, 10, or 20 had higher development scores than embryos cultured singly. The highest development score was obtained by the combination of 5 embryos per 10-µl drop. The percentage of live embryos in 20 or 40 µl was lower than that of embryos cultured in 10 µl. Additionally, the percentage of live embryos cultured singly was lower than that of embryos cultured in groups.
    Notes: Background One-cell mouse embryos were used to determine the effects of drop size and number of embryos per drop for optimum development in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01204051
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro preimplantation mouse embryo development with incubation temperatures of 37 and 39°C (1992)
    Springer
    Journal of assisted reproduction and genetics 9 (1992), S. 149-154 
    Details
    ISSN: 1573-7330
    Keywords: embryo culture ; incubation temperatures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Embryos from two strains of mice were used to assess the effect of incubation temperature on pronuclear and twocell development to the morula/blastocyst (M/B) stage. Embryos from B6D2F2 and B6SJLF1 strains were cultured in medium M16 at either 37 or 39°C until 120 hr post human chorionic gonadotropin (hCG) or 0, 24, or 48 hr at 37°C and the remaining time at 39°C. Overall M/B development for pronuclear embryos was 0.6, 0, 32.3, and 52.4% for 0—96, 24—72, 48—48, and 96—0 hr at 37 and 39°C, respectively. Only 0—96 and 24—72 hr at 37 and 39°C were not different (P 〉0.10). Overall M/B development for two-cell embryos was 48.1, 78.1, and 98.0% for 0—72, 24—48, and 72—0 hr at 37 and 39°C, respectively. Percentage development at each time was different (P 〈.01) for each category. Additionally, the number of nuclei for morulae and blastocysts tended to be higher for embryos initiating culture at the two-cell stage compared to pronuclear embryos. The first cell cycle was most dramatically affected by a 2°C increase in incubator temperature. More advanced embryos can tolerate slight increases in incubator temperature more readily than pronuclear embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01203755
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Effectiveness of Menuzo's B2 Medium with Buffalo Rat Liver Cells for Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes (1998)
    Springer
    Journal of assisted reproduction and genetics 15 (1998), S. 50-53 
    Details
    ISSN: 1573-7330
    Keywords: bovine ; buffalo rat liver cells ; embryo culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Purpose: The effectiveness of two culture media on the development of bovine embryos in a buffalo rat liver (BRL) coculture system was investigated. Methods: Bovine oocytes were matured and fertilized in vitro, then cocultured, 25 per well, for 7 days in 500 μl of modified M199 or modified Menuzo's B2 medium over a BRL cell monolayer at 39°C in an atmosphere of 5% CO 2 in air. Medium 199 was modified by the addition of 10% of (v/v) fetal bovine serum (FBS), 9 mg/ml bovine serum albumin, 2 mM glycine, 1 mM alanine, and 0.1 mM nonessential amino acids (NEAA). Menuzo's B2 medium was modified by the addition of 10% (v/v) FBS, 1 mM alanine, and 0.1 mM NEAA. Results: Modified Menuzo's B2 medium improved embryo development to the morula or blastocyst stage compared to modified M199 (121/353, 34.3%, versus 99/362, 27.3%, P〈0.05). Conclusions: These results suggest that Menuzo's B2 medium with modifications in a BRL coculture system can provide a significant benefit for culture of early bovine embryos over the traditional use of medium 199.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022582422932
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    Paper (German National Licenses)
    Fulltext
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