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Digitale Medien

Potato tubers as a biofactory for recombinant antibodies (1998)

Artsaenko, Olga ; Kettig, Barbara ; Fiedler, Ulrike ; [weitere]

Springer

Molecular breeding 4 (1998), S. 313-319

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Details

ISSN: 1572-9788

Schlagwort(e): antibody ; endoplasmic reticulum ; phytofarming ; potato ; production ; scFv

Quelle: Springer Online Journal Archives 1860-2000

Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft

Notizen: Abstract Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 °C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1009676832273

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