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Characterization of ovarian follicular fluids of sheep, pigs and cows using proton nuclear magnetic resonance spectroscopy (1990)

Gosden, R. G. ; Sadler, I. H. ; Reed, D. ; [et al.]

Springer

Cellular and molecular life sciences 46 (1990), S. 1012-1015

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ISSN: 1420-9071

Keywords: Ovary ; follicular fluid ; NMR spectroscopy ; sheep ; pig ; cow

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Proton NMR spectra were produced for Graafian follicular fluids obtained by aspiration from sheep, pig and cow ovaries. The following low molecular mass, non-protein-bound metabolites were detected at concentrations exceeding 0.1 mM: acetate, alanine, creatinine/creatine, glycine, D-3-hydroxybutyrate, lactate, valine. Glucose was difficult to quantify and N-acetyl sugars gave a broad resonance at 2.06 ppm, presumably representing side-chains of glycoproteins. Ethanol was detected at up to millimolar concentrations in some specimens, though the physiological significance of this finding was not clear. The concentrations of all metabolites were comparable to those of plasma. These results have therefore shown that NMR spectroscopy is useful for gaining a broad and semiquantitative impression of the more abundant metabolites in the fluids of preovulatory Graafian follicles.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01940658

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Capacitation potential of the fallopian tube: A study involving surgical insemination and the subsequent incidence of polyspermy (1988)

Hunter, R. H. F. ; Nichol, R.

New York, NY : Wiley-Blackwell

Gamete Research 21 (1988), S. 255-266

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ISSN: 0148-7280

Keywords: pig ; fertilization ; vitellus ; chromatin aggregates ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: In an attempt to demonstrate limitations in the capacitating potential of the Fallopian tube, ejaculated boar spermatozoa were introduced directly into the isthmus at varying intervals before ovulation. The incidence and degree of polyspermy subsequently observed were taken as indicators of the population of capacitated spermatozoa confronting the newly ovulated eggs: the more extensive the condition of polyspermy, the greater the number of capacitated spermatozoa presumed to have been available at the site of fertilization. Results are based on 673 eggs from 53 animals.When suspensions containing 2.21-3.87 × 108 sperm per ml were introduced 36-40 hours and 26-30 hours before ovulation, 85% and 61% respectively of the eggs were polyspermic, such eggs exhibiting mainly dispermy and trispermy. By contrast, when comparable sperm suspensions from the same boar were instilled 17-18 hours before ovulation, 70% of the eggs were polyspermic but the degree of polyspermy had increased dramatically: most eggs contained 40 or more sperm heads in the vitellus, invariably forming swollen chromatin aggregates rather than male pronuclei. Surgical insemination at times closer to ovulation significantly reduced the incidence and degree of polyspermy, reaching a low of 2% with insemination 1-2 hours before ovulation. These results therefore support the concept of a limited capacitation potential of the Fallopian tube.In a separate series of observations, mating animals shortly before surgical insemination with sperm suspensions from the same boar markedly reduced the incidence of polyspermy. This latter observation may be of clinical significance in procedures of laparoscopic or transcervical insemination into the tubes to alleviate human infertility. The manner whereby myosalpingeal physiology could be modified in response to coital stimulation is discussed.

Additional Material: 8 Ill.