ISSN:
1573-4994
Keywords:
Near-field
;
scanning near-field optical microscopy
;
fluorescence microscopy
;
three-dimensional imaging
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract Scanning near-field optical microscopy (SNOM) yields high-resolution topographic and optical information and constitutes an important new technique for visualizing biological systems. By coupling a spectrograph to a near-field microscope, we have been able to perform microspectroscopic measurements with a spatial resolution greatly exceeding that of the conventional optical microscope. Here we present SNOM images of Escherichia coli bacteria expressing a mutant green fluorescent protein (GFP), an important reporter molecule in cell, developmental, and molecular biology. Near-field emission spectra confirm that the fluorescence detected by SNOM arises from bacterially expressed GFP molecules.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1022554715488
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