ISSN:
1573-9368
Keywords:
gene construct
;
gene transfer
;
heritability
;
marker gene
;
pigmentation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transgenic mice provide a valuable tool in all fields of basic and applied biological and medical research. In this study, we describe the fate of integrated transgenes in the mammalian host genome over a large number of generations. The stability of the germ-line transmission of integrated tyrosinase transgene copies was monitored up to generation F20 in a large number of individuals from seven transgenic mouse lines. Phenotypic and molecular genetic analysis of the offspring both within the different lines and in cross-breeding experiments revealed the high stability of the transgene integration sites in mice. Only very few individuals were affected by a transgene copy loss. These results indicate that, once homozygous transgenic lines are established, breeding programs can be continued to a high number of generations without further stringent molecular genetic analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008824028100
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