ISSN:
1432-203X
Schlagwort(e):
Key words Agrobacterium rhizogenes
;
β-Glucuronidase
;
Daucus carota
;
gus gene
;
Root culture
;
NAM test
;
Opines
;
Gene expression
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Hairy root clones were established from carrot root discs inoculated with an agropine-type strain of Agrobacterium rhizogenes A4 harbouring the gus reporter gene on the TL-DNA. The clones were periodically examined for their phenotypic characteristics and for their ability to express the gus gene, to produce opines and to grow in the presence of NAM. The presence of the gus gene in the roots was confirmed by Southern blot hybridisation. The clones displayed various morphologies which were generally not correlated with the transformation events, and they were highly unstable throughout the successive subcultures, both for their phenotype and for their ability to express the transgenes. Reversible inactivation of the gus gene expression was associated with a high gus copy number. This could have some consequences for fundamental studies and practical uses of hairy roots.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002990050708
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