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  • hairy root  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Lobeline production by hairy root culture of Lobelia inflata L. (1990)
    Springer
    Plant cell reports 9 (1990), S. 307-310 
    Details
    ISSN: 1432-203X
    Keywords: Lobelia inflata L. ; hairy root ; lobeline ; root morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy roots were obtained following inoculation of the stems of Lobelia inflata L. with Agrobacterium rhizogenes strain ATCC 15834. These hairy roots contained agropine and mannopine. In addition, lobeline was detected by HPLC and confirmed by mass spectrometry. Various media were tested for the growth of hairy roots as well as for the content of lobeline in hairy roots. The growth rate of hairy roots cultured in Nitsch and Nitsch's medium was approximately one third of those cultured in other media. The lobeline content of hairy roots (18–54 μg/g dry weight) cultured in these media was the same order of magnitude compared with that of roots of L. inflata (24 μg/g dry weight) cultivated in pots. The hairy roots cultured in Nitsch and Nitsch's medium were morphologically different from those cultured in other media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232857
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An unusual root tip formation in hairy root culture of Hyoscyamus muticus (1994)
    Springer
    Plant cell reports 13 (1994), S. 349-352 
    Details
    ISSN: 1432-203X
    Keywords: Hyoscyamus muticus ; Agrobacterium rhizogenes ; hairy root ; adventitious root tip ; tropane alkaloid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hairy root cultures of Hyoscyamus muticus were established using Agrobacterium rhizogenes ATCC 15834. In one out of 8 clones established, an unusual root tip formation was observed after transfer of cultures from half-strength Murashige and Skoog (1962) to White's medium (1939). This phenomenon was associated with the production of a fine brownish cell suspension culture. Hairy root development resumed after transfer of the root tips from White to half-strength Murashige and Skoog medium. After plating the isolated brownish cells on hormone-free half-strength Murashige and Skoog or White solid medium, callus proliferation was observed, and then redifferentiation of hairy roots occurred. The polymerase chain reaction analysis of the H. muticus hairy root (clone Z2) revealed that only the tl region of the T-DNA was integrated. The growth and the production of five tropane alkaloids by this clone were examined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232635
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Traits of Panax ginseng hairy roots after cold storage and cryopreservation (1996)
    Springer
    Plant cell reports 15 (1996), S. 555-560 
    Details
    ISSN: 1432-203X
    Keywords: cold storage ; cryopreservation ; Panax ginseng ; hairy root ; ginsenoside
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Panax ginseng hairy root cultures were established by infecting petiole segments with Agrobacterium rhizogenes strain 15834. Hairy root segments including root tips placed onto phytohormone-free 1/2 Murashige and Skoog solid medium and stored at 4 °C in the dark for 4 months, resumed elongation when the temperature was raised to 25 °C in the dark. For cryopreservation, a vitrification method was applied. Root tips precultured with 0.1 mg/l 2,4-D for 3 days and dehydrated with PVS2 solution for 8 minutes prior to immersion into liquid nitrogen had a survival rate of 60 % and could regenerate. The hairy roots regenerated from cryopreserved root tips grew well and showed the same ginsenoside productivity and patterns as those of the control hairy roots cultured continuously at 25 °C. The conservation of T-DNAs in the regenerated hairy roots was proved by PCR analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232452
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    Paper (German National Licenses)
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