ISSN:
1432-1424
Keywords:
Bufo marinus
;
triiodothyronine
;
hormonal domain
;
aldosterone
;
sodium butyrate
;
transepithelial sodium transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary In the urinary bladder of the toadBufo marinus, the basal rate of synthesis of a number of proteins was modulated in a bidirectional way (i.e., induced or repressed) by aldosterone and by triiodothyronine (T3). Each hormone was therefore characterized by a distinct domain of response. When both hormones were added simultaneously, the two domains consistently overlapped at least for one protein, termed AIP-1, or aldosterone-induced protein 1 (M r≈65 kilodaltons,p i=6.7, as analyzed by two-dimension gel electrophoresis). The physiological role of AIP-1 is unknown, but could be related to the late mineralocorticoid response. In five experiments, T3 (60nm, 18-hr incubation) consistently repressed AIP-1, while aldosterone-dependent sodium transport (late response) was significantly inhibited, as previously described. The repression of AIP-1 was also observed as early as 6 hr after aldosterone addition. In addition, sodium butyrate (3mm), which was previously shown to also selectively inhibit the late mineralocorticoid response, was also able to repress AIP-1. Our results suggest that AIP-1, is one of the proteins involved in the mediation of the late mineralocorticoid response.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01869713
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