ISSN:
1573-7438
Keywords:
antigen
;
cattle
;
Hyalomma anatolicum anatolicum
;
immunization
;
immunoaffinity
;
larvae
;
protection
;
39 kDa protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Antigens from larvae of Hyalomma anatolicum anatolicum were extracted and purified by immunoaffinity chromatography using immunoglobulin ligands from cross-bred animals immunized with soluble larval antigen. Affinity-purified antigen (Aff-TLE) and a total larval extract (TLE) were used to immunize cross-bred (Bos indicus×Bos taurus) cattle. The group immunized with Aff-TLE rejected 71.6% of larvae and 77.3% of nymphs. However, the rejection percentages were lower in the TLE-immunized group. No significant changes in the feeding period, moulting percentages or moulting period of engorged larvae and nymphs were recorded. There was, however, a significant decrease in the number of resultant nymphs p〈0.01) and adults (p〈0.01) in the ticks fed on the Aff-TLE-immunized group. The Aff-TLE antigen was 93.3% purified. SDS-PAGE analysis identified a 39 kDa protein, reported for the first time, as the antigen responsible for the induction of resistance in the host.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005218821889
Permalink
