ISSN:
1432-0428
Schlagwort(e):
Fetal pancreas
;
morphology
;
insulin secretion
;
secretagogues
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary Whole fetal human pancreases of 12–22 weeks gestation, showed histological growth and differentiation in vitro over 3 weeks. At glucose concentrations of 1–4 g/l, there was no difference in insulin secretion into culture medium over 1 h. There was no stimulation of insulin release by D-glyceraldehyde, thus defective glucose-stimulated insulin release was probably not due to impairment of an early step in glycolysis. In the presence of 0.5 mmol/l dibutyryl cyclic AMP, insulin secretion was enhanced (0.188±0.030 versus 0.100±0.012 mU·mg tissue-1·h-1, p〈0.001) independently of glucose concentrations. It thus appears that impairment of glucose-stimulated insulin release was unlikely to be due to insufficient intracellular cyclic AMP. Insulin release increased in response to tolbutamide and theophylline. Insulin secretion was stimulated in the presence of a fivefold increase in amino acid concentration (0.118±0.018 versus 0.031±0.008 mU·mg tissue -1·h-1, p〈0.001). There was a fourfold increase in basal insulin secretion from islets previously grown in high concentration of amino acids compared with standard culture medium, (0.284±0.052 versus 0.067±0.011 mU·mg tissue-1·h-1, p〈0.001), emphasizing the important role of amino acids as substrates for B cell metabolism and development.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00260956
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