ISSN:
1432-0428
Keywords:
Insulin receptor
;
internalization
;
endocytosis
;
hepatocytes
;
binding
;
insulin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary By quantitative electron microscopic autoradiographic technique, we have previously shown that125I-insulin initially localizes to the plasma membrane of isolated rat hepatocytes and is subsequently internalized in a limited region of the peripheral cytoplasm. In the present study, we have shown that when cells are incubated at 20 °C, steady state binding is reached by 60 minutes and maintained up until 120 minutes of incubation while at 37 °C steady state binding is reached by 10 minutes and maintained for 30 minutes. Under both of these conditions, internalization of the labelled material occurs as a constant function of the binding. These data suggest that under normal conditions the binding of the ligand is an important rate limiting determinant of the internalization process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01236273
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