ISSN:
1420-9071
Keywords:
Juvenile hormone esterase
;
juvenile hormone epoxide hydrolase
;
juvenile hormone binding protein
;
reversed-phase liquid chromatography assay
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract We studied time-dependent metabolism of (10R)-[3H] juvenile hormone (JH) III and (10R, 11S)-[3H]JH I injected intoManduca sexta larvae; the hormones are metabolized to polar metabolites, expecially the JH acid-diol, and an unknown. Products were analyzed using a reversed-phase liquid chromatography assay. (10R)-JH III is metabolized much more rapidly than (10R, 11S)-[3H]JH I, whether injected seperately or as a mixture of hormones. The unknown metabolites of JH I and JH III were identified as phosphate conjugates of JH I and JH III diol by tandem mass spectral analysis of isolated samples. The phosphate conjugate of JH I diol is the principle end product of JH I metabolism.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02125646
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