ISSN:
1420-9071
Keywords:
Differentiation
;
glycosaminoglycan sulfation
;
protein kinase C
;
phorbol esters
;
Caco-2 cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4β-phorbol 12β-myristate, 13α-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4α-phorbol 12, 13 didecanoate was ineffective. These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01990515
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