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Formation of colonies of P-388 leukemic cells in semisolid agar (1978)

Ivanitskaya, L. P. ; Bibikova, M. V. ; Egorov, L. V. ; [et al.]

Springer

Bulletin of experimental biology and medicine 86 (1978), S. 973-975

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ISSN: 1573-8221

Keywords: leukemic cells ; cloning

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract By alternate passages of P-388 mouse ascites leukemia cells in a primary agarized culture and intraperitoneally in mice a substrain P-388/A2 adapted to culture in agar gel in the form of compact colonies without the use of special conditions of colony-stimulating factors was obtained. Reseeding efficiency and size of the colonies depended on the original density of the cell suspension. After the addition of 100 cells/ml seeding efficiency reached 20% and the number of cells in the colony by the 8th–10th day of culture was 13,000.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00801329

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