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Digitale Medien

Indirect Fluorimetric Detection of Cyclodextrins via Postcolumn Mixing with 2-p-Toluidinyl-6-naphthalenesulfonate in Liquid Chromatography (1999)

Takeuchi, Toyohide ; Miwa, Tomoo

Weinheim : Wiley-Blackwell

Journal of High Resolution Chromatography 22 (1999), S. 609-612

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ISSN: 0935-6304

Schlagwort(e): Cyclodextrins ; 2-p-toluidinyl-6-naphthalenesulfonate ; indirect fluorimetric detection ; liquid chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: ---Cyclodextrins (CD's) were visualized by postcolumn mixing with 2-p-toluidinylnaphthalene sulfonate (TNS) in liquid chromatography. The indirect detection is based on fluorescence enhancement due to the formation of an inclusion complex between TNS and CD's. β-CD gave larger signal intensity than α- and γ-CD. Different selectivities were observed for alkyl-bonded silica stationary phases with different chain length.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

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Digitale Medien

Separation and fluorescence enhancement of dansyl amino acids assisted by γ-cyclodextrin and bovine serum albumin as mobile phase additive in microcolumn liquid chromatography (1995)

Takeuchi, Toyohide ; Miwa, Tomoo ; Chu, Jianhua

New York, NY [u.a.] : Wiley-Blackwell

Journal of Microcolumn Separations 7 (1995), S. 575-580

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ISSN: 1040-7685

Schlagwort(e): liquid chromatography ; γ-cyclodextrin ; bovine serum albumin ; fluorescence enhancement ; retention behavior ; dansyl amino acids ; Chemistry ; Analytical Chemistry and Spectroscopy

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Chemie und Pharmazie

Notizen: The effects of γ-cyclodextrin and bovine serum albumin as mobile phase additives on fluorescence intensity and retention of dansyl amino acids (Dns-AAs) have been examined in microcolumn liquid chromatography. Polymercoated mixed-functional silica packings were employed for the separation column. The concentrations of the additives, acetonitrile, and buffer affected the signal intensity and retention of DNA-AAs. The use of the mobile phase additives improved the detection limits by a factor of as much as 7.4. © 1995 John Wiley & Sons, Inc.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/mcs.1220070604

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