ISSN:
1435-1463
Keywords:
Isobutanol bovine pineal extracts
;
compensatory ovarian hypertrophy (COH)
;
paper chromatography
;
high pressure
;
reverse phase
;
liquid chromatography (HPRPLC)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Bovine pineal glands were extracted according to the methods reported byBensinger et al. (1973) andCheesman andFariss (1970). Isobutanol soluble COH-inhibiting activity was further separated by chromatography on Sephadex G-15 and paper chromatography. With the Bensinger method, different active pineal fractions were obtained from Sephadex G-15 columns. Certain of those fractions were further separated by paper chromatography in butanol: acetic acid: water (4∶1∶1) and the COH-inhibitor was localized. The pineal COH-inhibitor could also be localized by high pressure, reverse phase liquid chromatography. More COH-inhibiting activity was extracted with the Bensinger method than with aqueous and acetic acid extraction methods used earlier by us. The Cheesman extraction method for arginine vasotocin gave less regular results in our hands than the Bensinger extraction method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01243880
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