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  • mass-spectrometry  (2)
  • respiration  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 243-251 
    ISSN: 1573-5044
    Schlagwort(e): photomixotrophy ; Euphorbia characias L. ; cell suspension ; oxygen exchange ; photosynthesis ; respiration ; mass-spectrometry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Growth of photomixotrophic cell culture of Euphorbia characias L. is described; oxygen exchange rates were measured along this growth cycle using a mass-spectrometric technique. During the exponential and mid-stationary phases, photosynthesis was strongly increasing, the major part of oxygen uptake in the light was due to mitochondrial respiration. In the late stationary phase, gross photosynthesis was decreasing; this could not be explained by an alteration of Rubisco because photorespiratory process could be observed; in addition the Mehler reaction must be called upon to explain whole oxygen uptake in the light.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 1573-5044
    Schlagwort(e): cell division ; Euphorbia characias ; gamma-irradiation ; mass-spectrometry ; oxygen exchange ; sucrose
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Gamma-irradiation (250 Gy) applied to photoautotrophic cell suspensions of Euphorbia characias L. in the exponential growth phase led to the arrest of cell division and to a subsequent overaccumulation of sucrose and dry matter. From the fourth day of culture, the chlorophyll content and gross photosynthesis were not depressed by gamma-treatment nor by sugar accumulation. In both cultures, no difference was observed between oxygen uptake in the light at CO2 saturating concentration and in the dark, suggesting that no change in energy-dissipative reactions took place after irradiation. A slight increase in oxygen uptake in both light and dark was observed in irradiated cells during the first four days. However, in the absence of limiting factors, the photosynthetic capacities of the dividing and irradiated non-dividing photoautotrophic cells were identical but higher than that of the non-dividing cells in the stationary growth phase. This suggests that gamma-irradiation arrests cell division by a mechanism different to that occuring in stationary-phase cultures. This may be of value in investigating the metabolism of secondary products.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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