ISSN:
1573-5044
Keywords:
Cell culture
;
Hypericum brasiliense
;
medicinal plant
;
micropropagation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Hypericum brasiliense, a non-domesticated plant has been shown to have useful medicinal properties. This plant has not been cultivated so a protocol for mass propagation based on selection of superior clones was developed and a protocol established for the culture of callus cells that could be used for in vitro metabolite production. A micropropagation method based on amplification of nodal buds was developed, by selection, from ten seedling clones that were examined for growth rate, multiplication rate and rooting. The effect of various basal media, growth regulator types and concentrations were examined for optimal callus induction. Optimal callus induction occured on either Murashige and Skoog's or Gamborg's media supplemented with 1 to 2 mg l−1 of 2,4-dichlorophenoxyacetic acid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00048184
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