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  • 1
    Electronic Resource
    Electronic Resource
    Specific insulin binding sites in snail (Helix aspersa) ganglia (1989)
    Springer
    Cellular and molecular neurobiology 9 (1989), S. 273-279 
    Details
    ISSN: 1573-6830
    Keywords: insulin ; insulin receptors ; neuropeptide receptors ; invertebrate nervous system ; invertebrate ganglia ; quantitative autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Insulin binding sites were characterized and quantified in snail (Helix aspersa) ganglia by incubation of tissue sections with125I-porcine insulin, autoradiography with [3H]Ultrofilm, image analysis coupled to computer-assisted microdensitometry, and comparison with125I-standards. Cellular localization was performed in the same sections by emulsion autoradiography. 2. Specific insulin binding sites were demonstrated in discretely localized groups of neurons of the cerebral, pleural, parietal, visceral, and pedal ganglia and in nerves. Scatchard analysis performed with consecutive sections from single animals revealed a single class of high-affinity insulin binding sites (K d, 0.13 ± 0.01 nM;B max, 157 ± 10 fmol/mg protein). 3. Our results suggest that insulin may play a role as a neurotransmitter or neuromodulator in snail ganglia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00713034
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitative autoradiographic localization of neuropeptide Y (NPY) binding sites in rat posterior pituitary lobe (1988)
    Springer
    Cellular and molecular neurobiology 8 (1988), S. 333-338 
    Details
    ISSN: 1573-6830
    Keywords: neuropeptide Y ; neuropeptide receptors ; pituitary gland ; posterior pituitary lobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. We have localized and quantified neuropeptide Y (NPY) binding sites in the rat pituitary gland after incubation of tissue sections in the presence of125I-Bolton-Hunter NPY followed by autoradiography, computerized microden-sitometry, and comparison to125I-standards. 2. In the rat, NPY binding sites are localized exclusively to the part of the posterior pituitary lobe closer to the pituitary stalk. No NPY binding sites could be found in the intermediate or the anterior pituitary lobes. 3. Our results suggest a role for NPY in the regulation of pituitary function and, in particular, that of the neural lobe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711175
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Estradiol increases dopamine turnover in intermediate and posterior pituitary lobes of ovariectomized rats (1984)
    Springer
    Cellular and molecular neurobiology 4 (1984), S. 397-402 
    Details
    ISSN: 1573-6830
    Keywords: estradiol ; dopamine turnover ; norepinephrine ; posterior pituitary lobe ; intermediate pituitary lobe ; rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Dopamine levels were specifically increased in the intermediate lobe of the pituitary gland of ovariectomized rats following acute 17-β-estradiol administration. 2. Estradiol treatment increased the dopamine turnover rate 10-fold in the intermediate lobe and 2-fold in the posterior lobe of ovariectomized rats. 3. In contrast, estrogen treatment had no effect on the endogenous levels or the turnover rate of norepinephrine in these tissues. 4. Our results suggest that estrogens can selectively modulate dopamine metabolism in the posterior and intermediate lobes of the rat pituitary gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00733600
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative autoradiographic characterization of receptors for angiotensin II and other neuropeptides in individual brain nuclei and peripheral tissues from single rats (1985)
    Springer
    Cellular and molecular neurobiology 5 (1985), S. 211-222 
    Details
    ISSN: 1573-6830
    Keywords: quantitative autoradiography ; receptor autoradiography ; neuropeptide receptors ; angiotensin II receptors ; receptor quantitation in brain nuclei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Autoradiographic techniques coupled with computerized microdensitometry and comparison with125I standards were used to characterize and quantitate receptors for neuropeptides in rat brain and adrenal and pituitary glands. 2. These techniques are rapidly performed, anatomically precise, and more sensitive than membrane binding techniques. They permit the determination of complete saturation curves and Scatchard analysis in discrete nuclei of the rat brain and in single rat pituitary and adrenal glands. 3. Angiotensin II (AII) receptors were quantitated after incubation of 16-µm tissue sections with the AII agonist125I-[Sar1]-AII. 4. High-affinity, high-density AII receptors were present in the organon subfornicalis, organon vasculosum laminae terminalis and nuclei triangularis septalis, suprachiasmatis, and paraventricularis of the rat and in rat adrenal capsule-zona glomerulosa area, adrenal medulla, and anterior pituitary. 5. These techniques could be used for precise localization and quantitation of other neuropeptide receptors in single rat brain nuclei, after optimizing the assay conditions and provided that suitable125I ligands are available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711007
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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