ISSN:
1573-5028
Keywords:
Agrobacterium rhizogenes-transformed root cultures
;
Nicotiana rustica
;
nicotine
;
ornithine decarboxylase
;
putrescine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout the growth cycle of some lines, as demonstrated by the analysis of mRNA and enzyme activity. The presence of the yeast gene and enhanced ornithine decarboxylase activity is associated with an enhanced capacity of cultures to accumulate both putrescine and the putrescine-derived alkaloid, nicotine. Even, however, with the very powerful promoter used in this work the magnitude of the changes seen is typically only in the order of 2-fold, suggesting that regulatory factors exist which limit the potential increase in metabolic flux caused by these manipulations. Nevertheless, it is demonstrated that flux through a pathway to a plant secondary product can be elevated by means of genetic manipulation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00017721
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