ISSN:
1573-6830
Keywords:
nitric oxide
;
N G-methyl-L-arginine
;
endothelin-3
;
dopamine release
;
neurotoxicity
;
rat striatal slices
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract 1. Our method of real-time monitoring of dopamine release from rat striatal slices revealed that endothelin (ET)-3-induced dopamine release was inhibited by N G-methyl-L-arginine (L-NMMA; 1 mM), an inhibitor of nitric oxide (NO) synthase, while N G-methyl-D-arginine (D-NMMA; 1 mM), an inactive isomer of L-NMMA, had no effect. 2. The inhibition of L-NMMA (0.1 mM) became apparent when tissues were pretreated with tetrodotoxin (1 μM) for 30 min and subsequently exposed to ET-3 (4 μM). 3. L-NMMA (0.1 and 1 mM) dose dependently protected against ET-3-triggered hypoxic/hypoglycemic impairment of striatal responses to high K+. 4. Thus, NO may work as a promoter in mediation of the stimulatory and neurotoxic action of ET-3 on the striatal dopaminergic system, presumably by interacting with interneurons in the striatum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026354720732
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