ISSN:
0173-0835
Keywords:
Capillary zone electrophoresis
;
Indirect VIS detection
;
Carbohydrates
;
Borate complexation
;
p-Nitrophenol
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Borate complexation was used to make possible the separation of disaccharides by capillary electrophoresis with indirect detection. A high borate concentration did not affect the indirect detection sensitivity in as negative a way as predicted previously. The concentration sensitivity for sucrose was determined to be 2 mM at the borate concentration of 200 mM in running electrolyte. The newly introduced backround chromophore, p-nitrophenol, allows the monitoring of the separation process in a visible range at 400 nm. This also enables the indirect detection of UV-absorbing compounds in complex mixtures in which they would be impossible to detect with a UV-absorbing backround chromophore.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150180721
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