ISSN:
1573-4943
Keywords:
Manual Edman degradation
;
phosphoprotein analysis
;
trifluoroethyl isothiocyanate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A manual Edman degradation protocol has been developed that allows the identification of phosphorylation sites in 32P-labeled peptides at the subpicomole level. By using both a volatile reagent, trifluoroethyl isothiocyanate, and volatile buffers, extraction steps are rendered unnecessary and cycle times can be reduced to 45 min. The protocol was employed to identify the site of phosphorylation in phosphoserine- and phosphotyrosine-containing peptides.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026368201651
Permalink