ISSN:
1432-203X
Keywords:
Nicotiana tabacum L. cv. xanthi
;
N. plumbaginifolia Viv.
;
Glycine canescens F. J. Herm.
;
Daucus carota L. var sativa
;
Solanum tuberosum L. cv. Merrimac
;
Datura innoxia Mill.
;
benomyl
;
fungicide
;
plant cell culture
;
protoplast culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The systemic fungicide methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate (benomyl), is a broad spectrum fungicide. Benomyl at concentrations up to 50 mg/l does not inhibit the growth of suspension cultures ofNicotiana tabacum, Datura innoxia, Daucus carota, Glycine canescens, andSolanum tuberosum nor growth ofN. tabacum orN. plumbaginifolia protoplasts if benomyl is dissolved by autoclaving or boiling. Addition of benomyl dissolved in dimethyl sulfoxide results in a visible toxicity. Benomyl, at 6.25–50 mg/l preventsPenicillium spp. growth in both protoplast and cell cultures and can be used to remove fungal contaminates after one to three transfers without visibly retarding plant cell growth. Due to the broad spectrum of fungicidal activity, and nontoxicity at high concentrations when dissolved by boiling or autoclaving, benomyl can be used effectively to control or prevent fungal contamination in plant cell and protoplast cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00571298
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