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  • 1
    Electronic Resource
    Electronic Resource
    Plant regeneration from protoplasts of wild barley (Hordeum murinum L.) (1994)
    Springer
    Plant cell reports 13 (1994), S. 139-144 
    Details
    ISSN: 1432-203X
    Keywords: protoplast culture ; plant regeneration ; somatic embryogenesis ; Hordeum murinum L. ; wild resource
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have produced a large number of plants regenerated from protoplasts originally isolated from embryo-derived cell suspensions of wild barley, Hordeum murinum L.. Suspensions initially allowed protoplast isolation and culture 5.5 to 9 months from the date of callus initiation. Colony formation efficiencies ranged from 1.5 to 3.0 % and from 0.1 to 1.4 % for protoplast cultures with and without nurse cells, respectively. Both nurse and non-nurse techniques allowed efficient embryogenesis and plant regeneration. More than 400 shoots/plantlets have been obtained from 6 independent experiments. Over 150 plants have been transferred to the greenhouse. Protoplasts isolated from the youngest suspensions (5.5 months old) gave rise to the largest number of plants. Protoplasts isolated from suspensions as old as 15 months were also regenerable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239880
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Primary callus as source of totipotent barley (Hordeum vulgare L.) protoplasts (1996)
    Springer
    Plant cell reports 16 (1996), S. 137-141 
    Details
    ISSN: 1432-203X
    Keywords: Protoplasts ; Hordeum vulgare L. ; nurse culture ; plant regeneration ; primary callus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Primary callus of barley (Hordeum vulgare L.) derived from scutella (cv. ‘Dissa’) and anthers (cv. ‘Igri’) was used for protoplast isolation and plant regeneration. The protoplasts were embedded in agarose and cultured with nurse cells. The plating efficiency varied from 0.1% to 0.7%. Shoots regenerated from the developing callus. Plantlets were transferred to soil and cultivated in the greenhouse three to five months after protoplast isolation. All plants were normal in morphology, and most of them flowered and set seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01890854
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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