ISSN:
1573-6822
Keywords:
cell viability
;
in vitro assays
;
lipid peroxidation
;
lymphocytes
;
mono(ADP-ribose) transferase
;
poly(ADP-ribose) polymerase
;
sulfur mustard
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract This study was conducted to determine whether inhibitors of normal cellular functions can reduce cytotoxicity induced by sulfur mustard (HD). The compounds examined include inhibitors ofpoly(ADP-ribose) polymerase (PADPRP), inhibitors of mono(ADP-ribose) transferase (MADPRT), inhibitors of lipidperoxidation, and an inhibitor ofprotein synthesis. To determine the effects of these compounds on HD-induced cell death, human lymphocyte preparations were treated with known concentrations (0.1 μM to 1000 μM) of an inhibitor and exposed to an estimated 87% effect concentration (EC87) of HD (170 μM) for loss in cell viability. Cell viability was determined at 24–26hr post-exposure to HD using a dye (propidium iodide) exclusion assay and a flow cytometer. All of the selected PADPRP inhibitors were found to be effective at reducing the cytotoxic effects of HD. These inhibitors were rank-ordered based on the concentration that gives 50% (EC50) reduction ofHD-induced cell death.A signijicant correlation (r=0.94) was observed between the compounds' ability to inhibit PADPRP and the compounds' ability to reduce HD- induced cell death, suggesting that PADPRP plays a role in HD-induced cell death. Inhibitors of MADPRT, lipid peroxidation, and protein synthesis were not effective at reducing HD-induced cell death.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00757578
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