ISSN:
1573-904X
Keywords:
biodegradable microspheres
;
poly(D,L-lactide-co-glycolide)
;
tetanus toxoid
;
vaccine
;
antibody response
;
stabilizing agents
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Purpose. Antigenic proteins encapsulated in biodegradable polyester microspheres (MS) can slowly denature or aggregate, which results in decreased antigenicity. In this study, we have evaluated the ability of co-encapsulated additives to protect against the loss of tetanus toxoid (TT) antigenicity. Methods. Antibody responses were analyzed after immunization of mice with TT microencapsulated in the presence of additives (TT-MS-additive). Results. Immunization with TT-MS-additives gave rise to higher responses than those obtained in the absence of additive. BSA, trehalose, -γ-hydroxypropylcyclodextrin and calcium salts preserved the immunogenicity of the incorporated antigen with the highest efficacy. Sustained responses were obtained with mixtures of fast and slowly releasing TT-MS containing BSA plus trehalose or calcium salts. Conclusions. The selected additives may stabilize the antigen in MS during storage and rehydration in body fluids. Regulated antigen release from MS-based vaccines permits a reduction of the antigen dose and optimization of single-dose vaccine formulations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1011950732105
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