ISSN:
1573-5028
Keywords:
mRNA processing
;
Nicotiana
;
polymerase chain reaction
;
sequencing
;
transcription
;
yeast
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The xylanase gene from Cryptococcus albidus contains seven introns. Genomic and cDNA clones under the control of the CaMV 35S promoter were transferred into tobacco plants using Agrobacterium-mediated cell transformation. The genes were transcribed and the mRNAs were amplified by the polymerase chain reaction using primers on each side of the intron region. About 90% of the amplification products from plants transformed with the genomic clone corresponded to the size of the pre-mRNA (1.2 kb) and 10% represented the spliced product (0.85 kb). The 0.85 kb fragment was cloned and sequenced and the result indicated that the introns from the xylanase gene were accurately spliced by the plant cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040660
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