ISSN:
1573-5028
Keywords:
1-aminocyclopropane-1-carboxylic acid (ACC)
;
ACC synthase
;
S-adenosylmethionine (AdoMet)
;
ethylene
;
polymerase chain reaction (PCR)
;
Vigna radiata
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The polymerase chain reaction (PCR) was used to produce 3 putative clones for ACC synthase from etiolated mung bean (Vigna radiata Rwilcz cv. Berken) hypocotyls. This was accomplished by utilizing genomic DNA from mung bean and degenerate primers made from information derived from highly conserved regions of ACC synthase from different plant tissues. The total length of pMAC-1, pMAC-2 and pMAC-3 are 308, 321, and 326 bp, respectively, all of which code for 68 amino acids. The introns for pMAC-1, pMAC-2 and pMAC-3 are 92, 105, and 110 bp, respectively. The degrees of homology at the DNA level for each of these clones is ca. 80% in their coding region and ca. 50% in their respective introns. This is the first report providing evidence that there are at least 3 genes for ACC synthase in etiolated mung bean.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020022
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