ISSN:
1573-5028
Keywords:
chloroplast
;
Euglena gracilis Z
;
oxygen evolution
;
photosystem II
;
precursor protein
;
protein transport
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photosystem II (PS II) encoded by nuclear DNA is not a polyprotein. The precursor was identified as a 45 kDa protein by immunoprecipitation of in vitro translation products of mRNA and by a pulse-chase experiment. It is probable that the structure of the precursor of the nuclear-encoded protein in Euglena chloroplast is closely related to the feature of assembly, as well as of transport, of the protein in chloroplast.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00039627
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