ISSN:
1572-9818
Keywords:
GFP
;
GUS
;
internal standard
;
promoter
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Four different promoters (35S and enhanced 35S of the cauliflower mosaic virus, polyubiquitin of maize and actin1 of rice) were compared in a transient assay using maize leaves and particle bombardment. A gene encoding the jellyfish green fluorescent protein (GFP) driven by the 35S promoter was used as an internal standard to monitor the effectiveness of each bombardment. Normalisation of the transient expression assay using the GFP reference significantly reduced the variability between separate bombardments and allowed for a rapid and accurate evaluation of different promoters in microprojectile-bombarded leaves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007548813786
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