ISSN:
0192-253X
Keywords:
Dictyosteliurn discoideurn
;
develop- mentally regulated multigene family
;
spore germination
;
developmentally regulated genes
;
developmentally regulated proteins
;
proteins containing internal amino acid repeat
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Two different cDNA clones denoted pT0270-6 and pT0270-11 represent two mRNAs that are developmentally regulated during spore germination in Dictyosteliurn discoideum. The respective mRNAs are found only during early germination and are not present in other stages of growth or multicellular development. Four different genomic clones that hybridize to sequences that are common to both of the 270 cDNA clones were isolated from Dictyostelium libraries and se- quenced. Two are the genes for the two cDNAs, and the other two represent genes that do not seem to be transcribed. All four genomic se- quences possess a very unusual internal feature in the deduced protein sequences composed of a monotonous repeat of the tetrapeptide threonine- glutamic acid-threonine-proline. The other portions of the proteins have no homology among them- selves. The deduced protein corresponding to the 270-6 gene is very similar to avocado (Persea arnericana) cellulase. Since cellulose in the spore wall has to be digested during spore germination this suggests that this protein may function as an endo-( 1,4)-beta-D-glucanase during germination.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020120121
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