ISSN:
1432-0983
Keywords:
Transformation of Trichoderma viride
;
pyr4 gene of Neurospora crassa
;
Expression of the Taka-amylase A gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A pyrG mutant of Trichoderma viride, a very efficient cellulase producer, was isolated from among 5-fluoroorotic acid-resistant mutants. The mutation was complemented with the pyr4 gene of Neurospora crassa and used as a selection marker for the transformation of T. viride. A plasmid vector, pDJB1-Taa, carrying both the pyr4 gene and a gene encoding Taka-amylase A from Aspergillus oryzae, was constructed and introduced into protoplasts of T. viride pyrG-. The transformation frequency was 1–10 transformants (3 on average) per μg DNA. One transformant showed highly elevated α-amylase production (about 17 times higher than the recipient level) and the integration of more than one copy of the Taka-amylase gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00309916
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