ISSN:
1573-8221
Keywords:
avitaminosis B6
;
pyridoxine
;
pyridoxal
;
pyridoxal-5′-phosphate
;
cytolytic activity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The effect of different degrees of avitaminosis B6 in mice on the cytolytic activity of T lymphocytes, measured as the quantity of Na2Cr51O4 released from lysed target cells, was studied on a model of the primary immune response in a mixed lymphocyte culture in vitro. Keeping animals for 3 weeks on a diet without pyridoxine did not affect the ability of the lymphocytes to proliferate in vitro or their cytolytic activity. In animals receiving a diet without pyridoxine for 45 days the content of pyridoxal-5′-phosphate in the spleen was 55% lower than in the control. Lymphocytes taken from these animals, when cultured in vitro, showed sharply weakened ability to incorporate [3H]thymidine into DNA in response to the alloantigen. The cytolytic activity of these lymphocytes also was reduced. The ability of different forms of pyridoxine to restore the functions of T lymphocytes, when disturbed by avitaminosis B6, was studied.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00799201
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