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  • 1
    Electronic Resource
    Electronic Resource
    Intraspecific length heterogeneity of the rDNA-IGR in Arabidopsis thaliana due to homologous recombination (1993)
    Springer
    Plant molecular biology 22 (1993), S. 543-545 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; rDNA ; intergenic region ; spacer heterogeneity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called ‘C’ repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the observed length heterogeneity is due to homologous recombination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015983
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    25S-18S rDNA intergenic spacer ofNicotiana sylvestris (Solanaceae): Primary and secondary structure analysis (1999)
    Springer
    Plant systematics and evolution 218 (1999), S. 89-97 
    Details
    ISSN: 1615-6110
    Keywords: Solanaceae ; Nicotiana ; rDNA ; subrepeats ; molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete nucleotide sequence (3701 bp) of the intergenic spacer region between the 3′-end of 25S rRNA gene and the 5′-end of 18S rRNA gene (IGS) is described forNicotiana sylvestris. The IGS ofN. sylvestris consists of seven structural regions (SR) two of which are composed of subrepeats (SR II: three and one half copies of the 76 bp D-subrepeat and numerous short C-subrepeats; SR IV: five copies of the 142/136 bp A-subrepeat). A presumptive rRNA processing signal (CTTTT) is located at the beginning of SR I. An identical sequence is also present in each copy of the A-subrepeat. Each D-subrepeat in SR IIa contains at the 5′-end the conserved sequence (GAGGTTTTT) which may be involved in transcription termination. SR III (TG-rich) and SR IV (AT-rich) in the central part of IGS contain several potential replication origin sites. The transcription initiation site (TATATAAGGGGGG) is located at the border of SR IV and V. No spacer promoters have been found. SR V contains four copies of a CATGG motif that is also present in SR II, VI and VII. At the 5′-end of SR VII there are three copies of the 66 bp highly divergent B-subrepeat. Presumptive pre-rRNA processing signals are also present here. Possible hair-pin structures are described for SR V, VI and VII.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01087037
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular organization and evolution of the external transcribed rDNA spacer region in two diploid relatives ofNicotiana tabacum (Solanaceae) (1996)
    Springer
    Plant systematics and evolution 201 (1996), S. 117-129 
    Details
    ISSN: 1615-6110
    Keywords: Solanaceae ; Nicotiana ; rDNA ; external transcribed spacer ; phylogenetic subrepeat differentiation ; molecular evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For two close diploid relatives of the allotetraploidNicotiana tabacum, N. sylvestris (subg.Petunioides) andN. tomentosiformis (subg.Nicotiana), the nucleotide sequences of the external transcribed rDNA spacer regions (ETS) are described and compared (within the genus and with otherSolanaceae). The ETS of these two diploid species consists of three regions: the most upstream region I contains the putative RNA polymerase I transcription initiation site. Region II consists of several subrepeats (type A) and of a short species specific element. There are 5 subrepeats in theN. sylvestris clone and 10 subrepeats in the clone isolated fromN. tomentosiformis. Region III, adjacent to the 18S rRNA gene, exhibits much higher similarities between species than regions I and II, not only amongN. sylvestris andN. tomentosiformis but also with more distantly relatedSolanaceae. At the 5′-end of region III of the ETS in the twoNicotiana species studied in detail there are three copies of highly divergent subrepeats (type B). Possible ways of the step-wise molecular evolution of these ribosomal DNA regions inNicotiana and theSolanaceae are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989055
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    Paper (German National Licenses)
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