ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Scanning electron microscope (SEM) study of mouse embryos obtained from isolated blastomeres (1991)

Ponsà, M. ; Nogués, C. ; Vidal, F. ; [et al.]

Springer

Journal of assisted reproduction and genetics 8 (1991), S. 279-285

add to mindlist on the mindlist

Details

ISSN: 1573-7330

Keywords: scanning electron microscopy (SEM) ; embryos ; mouse ; isolated blastomeres

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract Preimplantation diagnosis and embryo sexing offer great possibilities in the prevention of human diseases and in the field of animal production. These techniques involve blastomere isolation. Isolated blastomeres can grow in culture and develop as whole embryos. In this paper we describe, at the scanning electron microscope level, the characteristics of the plasma membrane surface of isolated blastomeres obtained from mouse embryos at the two-cell stage and of embryos grown to the 2/4- and 4/8- cell stages and compare them to control embryos grown in vitro. According to our results the in vitro manipulation of these embryos does not affect the surface characteristics of the plasma membrane in the early cleavage stages.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01139785

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

Zona pellucida surface of immature and in vitro matured mouse oocytes: Analysis by scanning electron microscopy (1992)

Calafell, J. M. ; Nogués, C. ; Ponsà, M. ; [et al.]

Springer

Journal of assisted reproduction and genetics 9 (1992), S. 365-372

add to mindlist on the mindlist

Details

ISSN: 1573-7330

Keywords: scanning electron microscopy (SEM) ; zona pellucida ; oocyte in vitro maturation ; morphology

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Description / Table of Contents: Results Before in vitro maturation, the germinal vesicle immature group showed mainly an unstructured zona pellucida surface with smooth cumulus cells. The metaphase I immature group showed a more structured zona pellucida with smooth or blebbing cumulus cells. After in vitro maturation, development of the zona pellucida toward a mature surface, related to the initial degree of oocyte maturity, was observed in both groups.

Abstract: Conclusions These observations show a correlation between the morphology of the zona pellucida surface and the degree of oocyte maturity; the in vitro maturation process can give rise to a proper development of this endowment when immature oocytes are used.

Notes: Purpose The aim of this work was to determine the morphology of the zona pellucida surface of immature and in vitro matured mouse oocytes by scanning electron microscopy. For this purpose two groups of immature oocytes (germinal vesicle group and metaphase I group) were studied either before or after in vitro maturation.